Study on Antimicrobial Activity of Cotton Fabric Treated with Neem (Azadirachta indica A.Juss) Bark Extract
                                             The` Su Yee Tun1, Su Sandar Aung2, Kyi Pyar Win 3
                          Department of Textile Engineering, West Yangon Technological University
                                                     Yangon, Republic of the Union of Myanmar
                                                              [email protected]
This research is concerned with the investigation of the effect of neem stem bark extract on antimicrobial activity of cotton fabric. Antimicrobial agent is extracted from neem stem bark with ethanol by Soxhlet extraction method. Phytochemical screening of neem stem bark shows the presence of alkaloids, α-amino acid, terpenoids, glycosides, reducing sugar, phenols, saponins, carbohydrates. In these constituent compounds, alkaloids, glycosides, terpenoids, flavonoids and saponins are antibiotic principle of neem trees which are actually the defensive mechanism of the plants against different pathogens. Cotton fabric is treated with different concentrations of neem stem bark extract, (4 g/l, 6g/l and 8 g/l respectively) and citric acid is added to control the pH level of 4. Pad-dry-cure method is used for antimicrobial finishing treatment and curing is carried out at 110°C and 120°C, respectively. The impact of antimicrobial agent and finishing treatment on physical properties of treated cotton fabrics are also tested and analysed compared with untreated cotton fabric. After that, the effect of different concentrations of neem stem bark extract and curing temperatures on the antimicrobial activity of the treated fabrics against gram-positive (Staphylococcus aureus, Bacillus subtilis, Bacillus pumilus) and gram-negative (Pseudomonas aeruginosa, Candida albican, Eschelrichia coli) bacteria are investigated qualitatively by using agar-disc diffusion method. According to the test results, all the treated samples have shown acceptable antimicrobial activity against gram positive bacteria and gram negative bacteria.
Keywords – Neem stem bark, Soxhlet Extraction, Antimicrobial agent, Pad-dry-cure method, Agar disc diffusion